| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 221 | Acidithiobacillus ferrooxidans | ATAGACAAGCCCTGCGTGG | TGGCATCGCGTTCGTCAT | 59.78 | 60.13 | 165 | 93 |
100.00%
|
100.00%
|
| Primer 222 | Acidithiobacillus ferrooxidans | AACGATCTGCTGACCCTGC | AAAAGCGTGCCGTCGAGA | 60.08 | 59.97 | 227 | 92 |
100.00%
|
100.00%
|
| Primer 223 | Acidithiobacillus ferrooxidans | AGGAGGGTTGTCTGTCGGT | AAAAGCGTGCCGTCGAGA | 60.15 | 59.97 | 166 | 92 |
100.00%
|
100.00%
|
| Primer 224 | Acidithiobacillus ferrooxidans | AAAACGTGGCCATTCCGC | TGATGATCGCACGCTGCA | 59.35 | 60.13 | 285 | 92 |
100.00%
|
100.00%
|
| Primer 225 | Acidithiobacillus ferrooxidans | TTCACGTCATGGACCGCT | GGTTTGCAGCTTCTTCCGC | 58.94 | 60.08 | 188 | 92 |
100.00%
|
100.00%
|
| Primer 226 | Acidithiobacillus ferrooxidans | ACCCTCGATGACCTGCAGA | TGCACGCCAGCCAAATCT | 60.31 | 60.28 | 151 | 92 |
100.00%
|
100.00%
|
| Primer 227 | Acidithiobacillus ferrooxidans | TCTCCGGATGCGCAATGT | ACCTCACTGTCTCGGCAGA | 59.42 | 60.23 | 222 | 92 |
100.00%
|
100.00%
|
| Primer 228 | Acidithiobacillus ferrooxidans | TTATCGAGTCGCAGTGGCC | CCACTCGCACACTGACAGA | 59.86 | 59.64 | 289 | 92 |
100.00%
|
100.00%
|
| Primer 229 | Acidithiobacillus ferrooxidans | ATCACCGGTATGACCTGCG | TTCCTTGACCGCTGCGAT | 59.56 | 59.34 | 156 | 91 |
100.00%
|
100.00%
|
| Primer 230 | Acidithiobacillus ferrooxidans | TTCATCCCGTCGCTCATCG | TCAGCAAACGCAGGGTGA | 59.93 | 59.49 | 259 | 91 |
100.00%
|
100.00%
|